Article
Hydrogen production in nitrogenase mutants in Anabaena variabilis.
FEMS microbiology letters - 1 Mar 2010
Weyman Philip D, Pratte Brenda, Thiel Teresa
Abstract excerpt
Nitrogenase produces hydrogen as a normal byproduct of the reduction of dinitrogen to ammonia. The Nif2 nitrogenase in Anabaena variabilis is an alternative Mo-nitrogenase and is expressed in vegetative cells grown with fructose under strictly anaerobic conditions. We report here that the V75I substitution in the alpha-subunit of Nif2 showed greatly impaired acetylene reduction and reduced levels of (15)N(2)...
Topics
- Acetylene
- Amino Acid Sequence
- Anabaena variabilis
- Anaerobiosis
- Base Sequence
- Hydrogen
- Molecular Sequence Data
- Mutation
- Nitrogen Fixation
- Nitrogenase
- Oxidation-Reduction