Article
Genetic screening of newborns for sickle cell disease: correlation of DNA analysis with hemoglobin electrophoresis.
Clinical chemistry - 1 Mar 1991
Skogerboe K J, West S F, Murillo M D, Glass M W, Shaunak S, Tait J F
Abstract excerpt
Although DNA analysis based on the polymerase chain reaction (PCR) offers potential advantages for screening newborns for sickle cell disease, few data are available concerning the reliability of PCR-based tests for such screening. We describe a protocol for detecting the A, S, and C alleles of the beta-globin gene in dried blood from phenylketonuria screening cards. This method is based on PCR and detection with...
Topics
- Alleles
- Anemia, Sickle Cell
- DNA
- Electrophoresis
- Fetal Blood
- Globins
- Hemoglobins
- Humans
- Infant, Newborn
- Polymerase Chain Reaction