Article
The conserved GTPase center and variable region V9 from Saccharomyces cerevisiae 26S rRNA can be replaced by their equivalents from other prokaryotes or eukaryotes without detectable loss of ribosomal function.
Proceedings of the National Academy of Sciences of the United States of America - 15 Feb 1991
Musters W, Conçalves P M, Boon K, Raué H A, van Heerikhuizen H, Planta R J
Abstract excerpt
Using the "tagged" rRNA gene system, which allows in vivo mutational analysis of Saccharomyces cerevisiae rRNA, we studied the role of two distinct structural elements of 26S rRNA in ribosome biogenesis and function--namely, the evolutionarily highly conserved "GTPase center" located in domain II and the eukaroyote-specific variable region V9 in domain III. Replacement of the S. cerevisiae GTPase center with its...
Topics
- Escherichia coli
- GTP Phosphohydrolases
- Genetic Variation
- Macromolecular Substances
- Models, Molecular
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Plasmids
- Polyribosomes