[Hepatitis C virus genotyping: comparison of the Abbott RealTime HCV Genotype II assay and NS5B sequencing].

doi:10.1016/j.patbio.2009.07.023

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Article

[Hepatitis C virus genotyping: comparison of the Abbott RealTime HCV Genotype II assay and NS5B sequencing].

Pathologie-biologie - 1 Apr 2010

Vaghefi P, Marchadier E, Dussaix E, Roque-Afonso A-M

Abstract excerpt

PURPOSE OF THE STUDY: Hepatitis C virus genotyping is needed for treatment decision and monitoring. The results of a genotyping assay based on real-time PCR and TaqMan chemistry were compared with the results of NS5B region sequencing. MATERIALS AND METHODS: One hundred and two sera (genotypes 1-6) were tested. Amplification and detection of viral RNA were performed with the Abbott RealTime HCV Genotype II assay...

Topics

Computer Systems
Genotype
Hepacivirus
Hepatitis C, Chronic
Humans
Polymerase Chain Reaction
Prospective Studies
RNA, Viral
Reagent Kits, Diagnostic
Sequence Analysis, RNA
Taq Polymerase
Time Factors
Viremia

Source

Open in PubMed

Identifiers

PMID: 19942365
DOI: 10.1016/j.patbio.2009.07.023

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