Article
15N-NMR characterization of His residues in and around the active site of FeSOD.
Biochimica et biophysica acta - 1 Feb 2010
Miller Anne-Frances, Yikilmaz Emine, Vathyam Surekha
Abstract excerpt
We have exploited (15)N-NMR to observe histidine (His) side chains in and around the active site of Fe-containing superoxide dismutase (FeSOD). In the oxidized state, we observe all the non-ligand His side chains and in the reduced state we can account for all the signals in the imidazole spectral region in terms of the non-ligand His', paramagnetically displaced signals from two backbone amides, and the side...
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