Article
Expression and self-assembly of virus-like particles from two genotypes of marine vesiviruses and development of an ELISA for the detection of antibodies.
Veterinary microbiology - 19 May 2010
McClenahan Shasta D, Bok Karin, Sosnovtsev Stanislav V, Neill John D, Burek Kathy A, Beckmen Kimberlee B, Smith Alvin W, Green Kim Y, Romero Carlos H
Abstract excerpt
Sequences encoding the major and minor capsid proteins (VP1 and VP2) from two marine vesivirus isolates (Steller sea lion viruses V810 and V1415) were engineered for expression of virus-like particles (VLPs) in the baculovirus system. The resulting VLPs were morphologically similar to native vesivirus virions. Purified VLPs were probed in immunoblots with pooled antisera specific for nine San Miguel sea lion...
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