Article
Identification and functional characterization of a gene for the methanol : N,N'-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803).
Microbiology (Reading, England) - 1 Feb 2010
Park Hyuk, Lee Hyunil, Ro Young T, Kim Young M
Abstract excerpt
Mycobacterium sp. strain JC1 is able to grow on methanol as a sole source of carbon and energy using methanol : N,N'-dimethyl-4-nitrosoaniline oxidoreductase (MDO) as a key enzyme for primary methanol oxidation. Purified MDO oxidizes ethanol and formaldehyde as well as methanol. The Mycobacterium sp. strain JC1 gene for MDO (mdo) was cloned, sequenced, and determined to have an open reading frame of 1272 bp....
Topics
- Alcohol Oxidoreductases
- Bacterial Proteins
- Blotting, Northern
- Carbon
- Catalysis
- Cloning, Molecular
- Energy Metabolism
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genetic Complementation Test
- Glucose