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Article

Lipase diversity in glacier soil based on analysis of metagenomic DNA fragments and cell culture.

Journal of microbiology and biotechnology - 1 Sept 2009

Yuhong Zhang, Shi Pengjun, Liu Wanli, Meng Kun, Bai Yingguo, Wang Guozeng, Zhan Zhichun, Yao Bin

Abstract excerpt

Lipase diversity in glacier soil was assessed by culture independent metagenomic DNA fragment screening and confirmed by cell culture experiments. A set of degenerate PCR primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly PCR amplify metagenomic DNA from glacier soil. These products were used to construct a lipase fragment clone library....

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PMID: 19809244
DOI: 10.4014/jmb.0812.695

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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