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Article

Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil.

Applied and environmental microbiology - 1 Mar 2003

Wery Nathalie, Gerike Ursula, Sharman Ajay, Chaudhuri Julian B, Hough David W, Danson Michael J

Abstract excerpt

Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10 degrees C and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests...

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Identifiers

PMID: 12620829
DOI: 10.1128/AEM.69.3.1457-1464.2003
PMCID: PMC150077

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