Article
Direct genotyping of cytomegalovirus envelope glycoproteins from toddler's saliva samples.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Dec 2009
Grosjean J, Hantz S, Cotin S, Baclet M C, Mengelle C, Trapes L, Virey B, Undreiner F, Brosset P, Pasquier C, Denis F, Alain S
Abstract excerpt
BACKGROUND: The polymorphism of genes encoding CMV envelope protein is used for strain classification and may influence pathogenesis and/or infectivity. CMV genotyping is usually based on sequencing or acrylamide gel-RFLP, but these methods are not suited to rapid screening of large populations. OBJECTIVES: We developed a high-throughput method to analyze CMV strains diversity and to detect multiple-strain...
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