Article
Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene.
Human genetics - 1 Nov 1990
Navajas M, Laurent A M, Moreel J F, Ragab A, Cambou J P, Cuny G, Cambien F, Roizès G
Abstract excerpt
The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed....
Topics
- Apolipoproteins B
- Base Sequence
- DNA
- Electrophoresis
- Humans
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length