[Function of human mutant CD59 molecule on diabetic vascular complication].

Abstract excerpt

AIM: To investigate the function of human mutant CD59 in diabetic pathology. METHODS: The recombinant PALTER-MAX plasmid containing human mutant CD59 cDNA and pcDNA plasmid were cotransfected into CHO cells using cation lipoid mediating method. Highly expressed cells were selected by flow cytometry and Western blot, which were then cultivated in high glucose. The functions of restricting complement membrane...

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