Article
Whole genome amplification and de novo assembly of single bacterial cells.
PloS one - 2 Sept 2009
Rodrigue Sébastien, Malmstrom Rex R, Berlin Aaron M, Birren Bruce W, Henn Matthew R, Chisholm Sallie W
Abstract excerpt
BACKGROUND: Single-cell genome sequencing has the potential to allow the in-depth exploration of the vast genetic diversity found in uncultured microbes. We used the marine cyanobacterium Prochlorococcus as a model system for addressing important challenges facing high-throughput whole genome amplification (WGA) and complete genome sequencing of individual cells. METHODOLOGY/PRINCIPAL FINDINGS: We describe a...
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