Article
Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis.
Journal of virology - 1 Oct 2009
Finkbeiner Stacy R, Li Yan, Ruone Susan, Conrardy Christina, Gregoricus Nicole, Toney Denise, Virgin Herbert W, Anderson Larry J, Vinjé Jan, Wang David, Tong Suxiang
Abstract excerpt
The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR)...
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