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Quantitative reverse transcription‐PCR assay for the rapid detection of methicillin‐resistant <i>Staphylococcus aureus</i>

13 Jul 2009

Abstract excerpt

AIM: To evaluate a new quantitative reverse transcription-PCR (qRT-PCR) assay for the rapid detection of methicillin-resistant Staphylococcus aureus (MRSA). METHODS AND RESULTS: Primers for Staphylococcus-specific regions of 16S rRNA gene, spa gene and mecA gene were newly designed. RNAs extracted from broth-cultured strains were tested by qRT-PCR targeting each primer, and the bacterial counts obtained...

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PMID: 19702857
DOI: 10.1111/j.1365-2672.2009.04476.x

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