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Rapid Detection of Methicillin-Resistant <i>Staphylococcus aureus</i> Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay

1 Jan 2003

Abstract excerpt

A rapid procedure was developed for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) directly from sterile sites or mixed flora samples (e.g., nose or inguinal swabs). After a rapid conditioning of samples, the method consists of two main steps: (i) immunomagnetic enrichment in S. aureus and (ii) amplification-detection profile on DNA extracts using multiplex quantitative PCR...

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PMID: 12517857
DOI: 10.1128/jcm.41.1.254-260.2003

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