Article
Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland.
Domestic animal endocrinology - 1 Jan 2010
Gal A, Ridge T K, Graves T K
Abstract excerpt
Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5...
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