Article
Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling.
Stem cells (Dayton, Ohio) - 1 Oct 2009
Kolle Gabriel, Ho Mirabelle, Zhou Qi, Chy Hun S, Krishnan Keerthana, Cloonan Nicole, Bertoncello Ivan, Laslett Andrew L, Grimmond Sean M
Abstract excerpt
Surface marker expression forms the basis for characterization and isolation of human embryonic stem cells (hESCs). Currently, there are few well-defined protein epitopes that definitively mark hESCs. Here we combine immunotranscriptional profiling of hESC lines with membrane-polysome translation state array analysis (TSAA) to determine the full set of genes encoding potential hESC surface marker proteins. Three...
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