Article
[Effects of different mutated sites in vpr gene of HIV on apoptosis of host cells: experiment with HeLa cells].
Zhonghua yi xue za zhi - 10 Mar 2009
Zheng Yu-huang, Zhang Chun-ying, He Yan, Gong Guo-zhong, Li Hui, Chen Zi, Liu Meng, Zhou Hua-ying, Li Ying, Liu Chun, Li Jing, Zhou Guo-qiang, Yin Wei, Yuan Hong-li
Abstract excerpt
OBJECTIVE: To investigate the effects of different mutated sites in the vpr gene of HIV on the apoptosis of host cells, and the possible mechanism thereof. METHODS: Fourteen HIV-1 vpr fragments were obtained from HIV-infected persons. Eukaryotic expression vector pcDNA3.1 (+) plasmid was extracted, the PCR purified product was double-cut by HindIII and BamH, and the cut products were ligated to vectors, thus...
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