Article
[Cloning and expression analysis of porcine ACTA2 gene and its association with production traits].
Yi chuan = Hereditas - 1 May 2009
Huang Jing-Shu, Xiong Yuan-Zhu, Deng Chang-Yan
Abstract excerpt
To identify new DNA markers which have significant impact on pig production traits, the full coding sequence and partial genomic sequence of porcine ACTA2(Actin alpha 2)gene were isolated using in silico cloning and PCR. PCR-Hinf-RFLP was developed to detect C1554T substitution in intron 2. The frequency of allele C is higher than that of allele T in all the seven detected pig populations except for Large White...
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