Article
In vivo RNA interference in oyster--vasa silencing inhibits germ cell development.
The FEBS journal - 1 May 2009
Fabioux Caroline, Corporeau Charlotte, Quillien Virgile, Favrel Pascal, Huvet Arnaud
Abstract excerpt
This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and...
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