Individual variability in post-thaw sperm survival in a captive koala population.

Abstract excerpt

This study documented the extent of individual animal variation with respect to two proven methods of sperm cryopreservation in a captive population of 22 koalas. Semen samples were collected by electroejaculation, diluted in Tris-citrate glucose and equilibrated to 4 degrees C before being further diluted and frozen in media containing a final concentration of either 14% (v/v) glycerol or 12.5% (v/v)...

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