The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope.

Abstract excerpt

Once the first methods for freezing mammalian semen had been established, research aimed at improving cryopreservation procedures became highly focused on the interactions between cooling rates and the permeability of the plasma membrane to water and cryoprotectants. This was based on the premise that cooling rates could be optimized from a theoretical basis for different species of interest. While this approach...

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