Article
Large scale comparative proteomics of a chloroplast Clp protease mutant reveals folding stress, altered protein homeostasis, and feedback regulation of metabolism.
Molecular & cellular proteomics : MCP - 1 Aug 2009
Zybailov Boris, Friso Giulia, Kim Jitae, Rudella Andrea, Rodríguez Verenice Ramírez, Asakura Yukari, Sun Qi, van Wijk Klaas J
Abstract excerpt
The clpr2-1 mutant is delayed in development due to reduction of the chloroplast ClpPR protease complex. To understand the role of Clp proteases in plastid biogenesis and homeostasis, leaf proteomes of young seedlings of clpr2-1 and wild type were compared using large scale mass spectrometry-based quantification using an LTQ-Orbitrap and spectral counting with significance determined by G-tests. Virtually only...
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