Article
A new strategy to improve a cauliflower mosaic virus vector.
Gene - 15 Sept 1991
Hirochika H, Hayashi K
Abstract excerpt
Co-infection of plants with non-overlapping deletion mutants of cauliflower mosaic virus usually leads to the production of the wild-type virus. To prevent this, a pair of mutants with overlapping deletions was constructed. In infected plants both mutant DNAs were stably maintained. Such mutants with overlapping deletions will be used as a vector to overcome the size limitation of genes to be cloned.
Topics
- Blotting, Southern
- Chromosome Deletion
- Cloning, Molecular
- DNA, Recombinant
- DNA, Viral
- Electrophoresis, Agar Gel
- Genetic Vectors
- Mosaic Viruses
- Mutation
- Plants
- Plasmids