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Article

Determination of AMP-activated protein kinase phosphorylation sites in recombinant protein expressed using the pET28a vector: a cautionary tale.

Protein expression and purification - 1 Aug 2009

Renz Bernhard, Davies Joanna K, Carling David, Watkins Hugh, Redwood Charles

Abstract excerpt

AMP-activated protein kinase (AMPK) is responsible for sensing of the cell's energetic status and it phosphorylates numerous substrates involved in anabolic and catabolic processes as well as interacting with signaling cascades. Mutations in the gene encoding the gamma 2 regulatory subunit have been shown to cause hypertrophic cardiomyopathy (HCM) with conduction abnormalities. As part of a study to examine the...

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Identifiers

PMID: 19269329
DOI: 10.1016/j.pep.2009.02.016
PMCID: PMC2691924

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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