Article
Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.
Journal of clinical microbiology - 1 May 2009
Hurst S F, Kidd S E, Morrissey C O, Snelders E, Melchers W J G, Castelli M V, Mellado E, Simmon K, Petti C A, Richardson S, Zhang S, Romanelli A M, Wickes B L, de Valk H A, Klaassen C H W, Balajee S Arunmozhi
Abstract excerpt
Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA,...
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