Article
Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique.
Journal of Zhejiang University. Science. B - 1 Feb 2009
Toral Ibañez Manuel, Caru Margarita, Herrera Miguel A, Gonzalez Luis, Martin Luis M, Miranda Jorge, Navarro-Cerrillo Rafael M
Abstract excerpt
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were...
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