Article
Practical considerations when using temperature to obtain rate constants and activation thermodynamics of enzymes with two catalytic steps: native and N460T-beta-galactosidase (E. coli) as examples.
The protein journal - 1 Feb 2009
Kappelhoff John C, Liu Su Yi Judy, Dugdale Megan L, Dymianiw Dayna L, Linton Larry R, Huber Reuben E
Abstract excerpt
The values of the rate constants and the associated enthalpies and entropies of enzymes with two catalytic steps can be measured by determining the effects of temperature on the k (cat) values. Practical considerations that should be taken into account when doing this are presented. The narrow te...
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