Molecular cloning and expression of rat liver 3 alpha-hydroxysteroid dehydrogenase.

doi:10.1210/mend-5-6-823

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Article

Molecular cloning and expression of rat liver 3 alpha-hydroxysteroid dehydrogenase.

Molecular endocrinology (Baltimore, Md.) - 1 Jun 1991

Cheng K C, White P C, Qin K N

Abstract excerpt

Complementary DNA clones encoding 3 alpha-hydroxysteroid dehydrogenase (3 alpha HSD) were isolated from a rat liver cDNA lambda gt11 expression library using monoclonal antibodies as probes. The sizes of the cDNA inserts ranged from 1.3-2.3 kilobases. Sequence analysis indicated that variation in the DNA size was due to heterogeneity in the length of 3' noncoding sequences. A full-length cDNA clone of 1286...

Topics

3-Hydroxysteroid Dehydrogenases
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cytosol
DNA
Escherichia coli
Gene Library
Genetic Variation
Genetic Vectors
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Kinetics

Liver

Molecular Sequence Data

Molecular Weight

Nucleic Acid Hybridization

Oligonucleotide Probes

Source

Open in PubMed

Identifiers

PMID: 1922097
DOI: 10.1210/mend-5-6-823

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1922097-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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