Article
Membrane protein frustration: protein incorporation into hydrophobic mismatched binary lipid mixtures.
Biophysical journal - 18 Feb 2009
Stopar David, Spruijt Ruud B, Hemminga Marcus A
Abstract excerpt
Bacteriophage M13 major coat protein was reconstituted in different nonmatching binary lipid mixtures composed of 14:1PC and 22:1PC lipid bilayers. Challenged by this lose-lose situation of hydrophobic mismatch, the protein-lipid interactions are monitored by CD and site-directed spin-label electron spin resonance spectroscopy of spin-labeled site-specific single cysteine mutants located in the C-terminal protein...
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