Article
Molecular cloning and gene expression analysis of tomato endo-beta-N-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose type free N-glycans during tomato fruit ripening.
Bioscience, biotechnology, and biochemistry - 1 Feb 2009
Nakamura Kosuke, Inoue Masami, Maeda Megumi, Nakano Ryohei, Hosoi Katsutoshi, Fujiyama Kazuhito, Kimura Yoshinobu
Abstract excerpt
In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the high-mannose type N-glycans with the Manalpha1-2Manalpha1-3Manbeta1-4GlcNAcbeta1-4GlcNAc unit. Real-time PCR analysis...
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