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Article

Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay.

Journal of virological methods - 1 May 2009

Hosaka Norimitsu, Ndembi Nicaise, Ishizaki Azumi, Kageyama Seiji, Numazaki Kei, Ichimura Hiroshi

Abstract excerpt

A rapid one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the pol-integrase gene was developed to detect human immunodeficiency virus type 1 (HIV-1) group M. This HIV-1 RT-LAMP assay is simple and rapid, and amplification can be completed within 35min under isothermal conditions at 60 degrees C. The 100% detection limit of HIV-1 RT-LAMP was determined using a standard...

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Source

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Identifiers

PMID: 19186193
DOI: 10.1016/j.jviromet.2009.01.004
PMCID: PMC7112927

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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