Article
[Construction and stress tolerance of trehalase mutant in Saccharomyces cerevisiae].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Oct 2008
Lv Ye, Xiao Dongguang, He Dongqin, Guo Xuewu
Abstract excerpt
OBJECTIVE: Accumulation of trehalose is critical in improving the stress tolerance of Saccharomyces cerevisiae. Two enzymes are capable of hydrolyzing trehalose: a neutral trehalase (NTH1) and an acidic trehalase (ATH1). We constructed trehalase disruption mutants to provide a basis for future commercial application. METHODS: To retain the accumulation of trehalose in yeast cell, we constructed diploid homozygous...
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