Article
Comparison of pulsed-field gel electrophoresis and three rep-PCR methods for evaluating the genetic relatedness of Stenotrophomonas maltophilia isolates.
Letters in applied microbiology - 1 Nov 2008
Lin C-W, Chiou C-S, Chang Y-C, Yang T-C
Abstract excerpt
AIMS: In this study, three facile repetitive-sequence PCR (rep-PCR) techniques have been compared with the pulsed-field gel electrophoresis (PFGE) method for differentiating the genetic relatedness of clinical Stenotrophomonas maltophilia isolates. METHODS AND RESULTS: The dendrograms of 20 S. maltophilia isolates were constructed based on the data obtained from PFGE and three PCR-based methods, i.e....
Topics
- Bacterial Typing Techniques
- Cluster Analysis
- DNA Fingerprinting
- DNA, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Gram-Negative Bacterial Infections
- Humans
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid