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Development and application of loop-mediated isothermal amplification for detecting the highly benzimidazole-resistant isolates in Sclerotinia sclerotiorum.

Scientific reports - 26 Nov 2015

Duan Ya Bing, Yang Ying, Wang Jian Xin, Liu Cong Chao, He Ling Ling, Zhou Ming Guo

Abstract excerpt

Resistance of benzimidazole fungicides is related to the point mutation of the β-tubulin gene in Sclerotinia sclerotiorum. The point mutation at codon 198 (GAG → GCG, E198A) occurs in more than 90% of field resistant populations in China. Traditional detection methods of benzimidazole-resistant mutants of S. sclerotiorum are time-consuming, tedious and inefficient. To establish a suitable and rapid detection of...

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PMID: 26606972
DOI: 10.1038/srep17278
PMCID: PMC4660316

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