Article
Regulation of Agrobacterium tumefaciens T-cyt gene expression in leaves of transgenic potato (Solanum tuberosum L. cv. Désirée) is strongly influenced by plant culture conditions.
Plant molecular biology - 1 Oct 1991
Dymock D, Risiott R, de Pater S, Lancaster J, Tillson P, Ooms G
Abstract excerpt
The promoter region of the Agrobacterium tumefaciens T-cyt gene was linked in a translational fusion to the coding DNA of the reporter gene uidA (for beta-glucuronidase or GUS protein; EC 3.2.1.31) and to nos 3' flanking DNA. The chimaeric gene was introduced by Agrobacterium transformation into potato (Solanum tuberosum L. cv. Désirée). In nine transgenic lines, the average GUS levels were highest in extracts...
Topics
- Agrobacterium tumefaciens
- Alkyl and Aryl Transferases
- Culture Techniques
- Gene Expression Regulation
- Glucuronidase
- Mutation
- Phenotype
- Plants, Genetically Modified
- Plasmids
- Promoter Regions, Genetic