Article
Phenotypically normal transgenic T-cyt tobacco plants as a model for the investigation of plant gene expression in response to phytohormonal stress.
Plant molecular biology - 1 Oct 1991
Yusibov V M, Il P C, Andrianov V M, Piruzian E S
Abstract excerpt
The tumour-inducing T-DNA gene 4 (T-cyt gene) of the nopaline Ti plasmid pTiC58 was cloned and introduced into tobacco cells by leaf disc transformation using Agrobacterium plasmid vectors. Tobacco shoots exposed to elevated cytokinin levels were unable to develop roots and lacked apical dominance. Using exogenously applied phytohormone manipulations we were able to regenerate morphologically normal transgenic...
Topics
- Agrobacterium tumefaciens
- Blotting, Northern
- Blotting, Southern
- Cloning, Molecular
- Conjugation, Genetic
- Cytokinins
- Escherichia coli
- Gene Expression Regulation
- Indoleacetic Acids
- Models, Biological