Article
[Dynamic variation of HIV-1 CRF07_BC env gene in early HIV-1 infection].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Aug 2008
Liu Sheng-Ya, Xing Hui, Zhang Yuan-Zhi, Ruan Yu-Hua, Quan Yu, Zhu Jia-Hong, Shao Yi-Ming
Abstract excerpt
OBJECTIVE: To understand the evolution of HIV-1 CRF07_BC envelope, we performed a longitudinal study on two patients during their early HIV-1 infection. METHODS: RNA was extracted from the plasma of the individuals and the C2-C5 fragments of the gp120 gene of HIV-1 were amplified by RT-PCR. Purified DNA segments were inserted into T easy vector and transformed into E. coli Top 10 competent cells. Positive clones...
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