Article
Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses.
Journal of virological methods - 1 Mar 2009
Pham Ngan Thi Kim, Trinh Quang Duy, Nguyen Tuan Anh, Dey Shuvra Kanti, Phan Tung Gia, Hoang Le Phuc, Khamrin Pattara, Maneekarn Niwat, Okitsu Shoko, Mizuguchi Masashi, Ushijima Hiroshi
Abstract excerpt
A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all...
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