Article
[Importance of 3T3 feeder layer to establish epithelial cultures from cell suspension obtained from corneo-scleral rims].
Arquivos brasileiros de oftalmologia - 1 Jan 2000
Cristovam Priscila Cardoso, Glória Maria Aparecida da, Melo Gustavo Barreto, Gomes José Alvaro Pereira
Abstract excerpt
PURPOSE: To evaluate the importance of the presence of 3T3 fibroblasts for establishing limbal epithelial cultures from cell suspension obtained from corneo-scleral rims (CSR). METHODS: Corneo-scleral rims from different donors (n=6) had their posterior stroma and endothelium stripped away. Each corneo-scleral rim was divided into three equal segments that were set up in tissue culture in three different...
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