Article
Demonstration of a functional requirement for the carbamate nitrogen of ribulosebisphosphate carboxylase/oxygenase by chemical rescue.
Biochemistry - 28 May 1991
Smith H B, Hartman F C
Abstract excerpt
Ribulosebisphosphate carboxylase/oxygenase is reversibly activated by the reaction of CO2 with a specific lysyl residue (Lys191 of the Rhodospirillum rubrum enzyme) to form a carbamate that coordinates an essential Mg2+ cation. Surprisingly, the Lys191----Cys mutant protein, in the presence of CO2 and Mg2+, exhibits tight binding of the reaction intermediate analogue 2-carboxyarabinitol bisphosphate [Smith, H....
Topics
- Binding Sites
- Carbamates
- Carbon Dioxide
- Kinetics
- Mutation
- Rhodospirillum rubrum
- Ribulose-Bisphosphate Carboxylase
- Structure-Activity Relationship
- Sulfonic Acids