Article
Molecular screening of Clostridium difficile toxins A and B genetic determinants and identification of mutant strains.
FEMS microbiology letters - 1 Feb 1991
McMillin D E, Muldrow L L, Leggette S J, Abdulahi Y, Ekanemesang U M
Abstract excerpt
Three separate sets of polymerase chain reaction primers were designed to specifically detect the presence of a toxin A gene fragment, a toxin B gene fragment, and the entire toxin B gene. In addition toxin gene fragments that were amplified from well characterized toxic strains were tagged fluorescently and used as hybridization probes to screen C. difficile strains. A survey of 37 toxic strains and 10 non-toxic...
Topics
- Animals
- Bacterial Proteins
- Bacterial Toxins
- Base Sequence
- Blotting, Western
- Clostridioides difficile
- DNA, Bacterial
- Enterotoxins
- Erythrocyte Membrane
- Molecular Sequence Data
- Mutation