Article
The histone H1-lacZ' fusion protein produced in Escherichia coli binds to the 5'-TTGGCAnnnTGCCAA-3' motif on DNA.
FEBS letters - 14 Jan 1991
Mannermaa R M, Oikarinen J
Abstract excerpt
The coding region of the chicken histone H1.03 gene was cloned to a bacterial expression vector, and the 291-amino acid H1-beta-galactosidase fusion protein was isolated after induction with IPTG. The fusion protein recognizes the 5'-TTGGCAnnnTGCCAA-3' motif on DNA. The H1 globular domain was ini...
Topics
- Animals
- Bacterial Proteins
- Base Sequence
- Chickens
- DNA
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Histones
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Recombinant Fusion Proteins
- Substrate Specificity
- beta-Galactosidase