Article
A new evaluation method for quantifying PI3K activity by HTRF assay.
Biochemical and biophysical research communications - 19 Dec 2008
Sugita Hironobu, Dan Shingo, Kong Dexin, Tomida Akihiro, Yamori Takao
Abstract excerpt
PIK3CA, coding a catalytic subunit of PI3K p110alpha, is frequently mutated in cancer. In previous studies, p110alpha with hotspot mutations such as E545K and H1047R were shown to be gain-of-function mutations. However, quantitative evaluation of these mutants was not well established. Recently, a new method for measuring PI3K activity using homogeneous time-resolved fluorescence (HTRF) has been developed. Using...
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