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Article

Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Dec 2008

Fernandez-Silva I, Eduardo I, Blanca J, Esteras C, Picó B, Nuez F, Arús P, Garcia-Mas J, Monforte Antonio José

Abstract excerpt

We report the development of 158 primer pairs flanking SSR motifs in genomic (gSSR) and EST (EST-SSR) melon sequences, all yielding polymorphic bands in melon germplasm, except one that was polymorphic only in Cucurbita species. A similar polymorphism level was found among EST-SSRs and gSSRs, between dimeric and trimeric EST-SSRs, and between EST-SSRs placed in the open reading frame or any of the 5'- or...

Topics

Chromosome Mapping
Cucumis melo
DNA Primers
DNA, Plant
Expressed Sequence Tags
Genome, Plant
Genotype
Minisatellite Repeats
Polymorphism, Genetic
Sequence Analysis, DNA

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PMID: 18806992
DOI: 10.1007/s00122-008-0883-3

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