Article
Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Oct 2008
Iniguez-Luy Federico L, Voort Amy V, Osborn Thomas C
Abstract excerpt
The traditional development of simple sequence repeat (SSR) or microsatellite markers by probe hybridization can be time-consuming and requires the use of specialized laboratory equipment. In this study, probe hybridization was circumvented by using sequence information on 3,500 genomic clones mainly from Brassica oleracea to identify di, tri, tetra and penta-nucleotide repeats. A total of 587 primer pairs...
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