Article
Serotype-specific detection of African horsesickness virus by real-time PCR and the influence of genetic variations.
Journal of virological methods - 1 Dec 2008
Koekemoer J J O
Abstract excerpt
Real-time PCR hybridization probe sets were tested for the specific detection of amplified genome segment 2 cDNA from all nine serotypes of African horsesickness virus (AHSV). The hybridization probes were derived from the sequences of genome segments 2 of the nine reference strains of the virus and were designed to have clearly distinguishable peak melting temperatures. Viral dsRNA from each of the serotypes was...
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