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Article

Serotype-specific detection of African horsesickness virus by real-time PCR and the influence of genetic variations.

Journal of virological methods - 1 Dec 2008

Koekemoer J J O

Abstract excerpt

Real-time PCR hybridization probe sets were tested for the specific detection of amplified genome segment 2 cDNA from all nine serotypes of African horsesickness virus (AHSV). The hybridization probes were derived from the sequences of genome segments 2 of the nine reference strains of the virus and were designed to have clearly distinguishable peak melting temperatures. Viral dsRNA from each of the serotypes was...

Topics

African Horse Sickness
African Horse Sickness Virus
Animals
Genetic Variation
Genotype
Oligonucleotide Probes
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Serotyping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 18793672
DOI: 10.1016/j.jviromet.2008.08.010

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