Article
Isolation of side population cells from endometrial cancer cells using a violet laser diode.
Human cell - 1 Jan 2014
Tomiyasu Satoshi, Miyamoto Tomoyuki, Mori Michihiro, Yaguchi Takahiro, Yakushiji Hiromasa, Ohno Setsuyo, Miyake Yasuyuki, Sakaguchi Takuya, Ueda Masatsugu, Ohno Eiji
Abstract excerpt
Cancer stem cells (CSCs) possess the ability for self-renewal, differentiation, and tumorigenesis and play a role in cancer recurrence and metastasis. CSCs are usually sorted in analysis into side population (SP) cells using ultraviolet (UV) laser (350 nm) excitation; they cannot be stained with Hoechst 33342 because of their efflux ability. However, it is difficult to avoid cell damage using a UV laser....
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