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Archaeal RNA polymerase subunits E and F are not required for transcription in vitro, but a Thermococcus kodakarensis mutant lacking subunit F is temperature-sensitive.

Molecular microbiology - 1 Nov 2008

Hirata Akira, Kanai Tamotsu, Santangelo Thomas J, Tajiri Momoko, Manabe Kenji, Reeve John N, Imanaka Tadayuki, Murakami Katsuhiko S

Abstract excerpt

All archaeal genomes encode RNA polymerase (RNAP) subunits E and F that share a common ancestry with the eukaryotic RNAP subunits A43 and A14 (Pol I), Rpb7 and Rpb4 (Pol II), and C25 and C17 (Pol III). By gene replacement, we have isolated archaeal mutants of Thermococcus kodakarensis with the subunit F-encoding gene (rpoF) deleted, but we were unable to isolate mutants lacking the subunit E-encoding gene (rpoE)....

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Identifiers

PMID: 18786148
DOI: 10.1111/j.1365-2958.2008.06430.x
PMCID: PMC3737576

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